Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EGR1

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa
cell line
HeLa
cell type
epithelial cell
chip antibody
EGR1
treatment
NT

Sequenced DNA Library

library_name
GSM6756518
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 15 minutes at room temperature. Samples for ChIP-seq were prepped as previously described (Cesana et al. 2018) Libraries were prepared from 10 ng of DNA using the NEBNext® Ultra™ II DNA Library Prep Kit following the manufacturer protocol

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
39869691
Reads aligned (%)
96.1
Duplicates removed (%)
9.6
Number of peaks
1316 (qval < 1E-05)

hg19

Number of total reads
39869691
Reads aligned (%)
95.3
Duplicates removed (%)
9.9
Number of peaks
965 (qval < 1E-05)

Base call quality data from DBCLS SRA