Antigen

Antigen Class

TFs and others

Antigen

EGR1

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa

cell line

HeLa

cell type

epithelial cell

chip antibody

EGR1

treatment

NT

Sequenced DNA Library

library_name

GSM6756518

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 15 minutes at room temperature. Samples for ChIP-seq were prepped as previously described (Cesana et al. 2018) Libraries were prepared from 10 ng of DNA using the NEBNext® Ultra™ II DNA Library Prep Kit following the manufacturer protocol

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

39869691

Reads aligned (%)

96.1

Duplicates removed (%)

9.6

Number of peaks

1316 (qval < 1E-05)

hg19

Number of total reads

39869691

Reads aligned (%)

95.3

Duplicates removed (%)

9.9

Number of peaks

965 (qval < 1E-05)